vectasheild hardset antifade mounting medium with dapi  (Vector Laboratories)

Journal: bioRxiv

Article Title: Viscoelasticity drives EMT in pancreatic intraepithelial neoplasia

doi: 10.64898/2026.02.11.705320

Figure Lengend Snippet: (a) Representative images of immunofluorescent staining for actin (green) and nuclei stained with DAPI (blue), from pancreatic epithelial cancer cells grown on hydrogels over 7 days for (V) samples (left), and quantification of cell area on all hydrogels across 7 days (right). (b) Cell morphology on hydrogels at 7 days; representative images of immunofluorescent staining for actin (green), and nuclei stained with DAPI (blue), from pancreatic epithelial cancer cells grown on all hydrogels at 7 days ( i ) with quantification of cell area (left) and circularity (right) ( ii ). (c) Schematic representation of morphological descriptors as a readout of EMT. Across all figures, n = 26-54 in which n represents individual cell measurements sampled from random areas on each hydrogel across three technical replicate samples per condition, error bars denote standard deviation, ** = p ≤ 0.01, **** = p ≤ 0.0001 derived from Kruskal-Wallis tests. Scale bars = 30 µm.

Article Snippet: After primary Ab incubations, samples were washed three times with 0.5 % Tween-20 prior to addition of secondary Ab solutions (diluted appropriately in 1% BSA) and incubation at room temperature in the dark for 1 h. Samples were then washed three times with 0.5 % Tween-20 before mounting onto glass slides using VECTASHEILD antifade mounting medium with DAPI (Vector Laboratories).

Techniques: Staining, Standard Deviation, Derivative Assay

Journal: bioRxiv

Article Title: Viscoelasticity drives EMT in pancreatic intraepithelial neoplasia

doi: 10.64898/2026.02.11.705320

Figure Lengend Snippet: (a) Characterisation of YAP localisation within pancreatic epithelial cancer cells after 3 days culture in response to altered hydrogel viscoelasticity. Representative images of immunostaining of cells with DAPI, or antibodies against actin (green) and YAP (red, left) and quantification of nuclear:cytoplasmic YAP ratios (right). (b) Characterisation of vimentin expression within pancreatic epithelial cancer cells after 3 days culture in response to altered hydrogel viscoelasticity. Representative images of cell immunostaining with DAPI, or antibodies against actin (green) and vimentin (red, left) and quantification of vimentin intensity normalised to healthy (E) sample (right). (c) Schematic to represent the role of viscoelastic cell mechanosensing during EMT in PanIN tissue through upregulation of YAP and vimentin. Across all figures, n = 24-33 in which n represents individual cell measurements sampled from random areas on each hydrogel across three technical replicate samples per condition, ** = p ≤ 0.01, *** = p ≤ 0.001 **** = p ≤ 0.0001 derived from Kruskal-Wallis ( i ) and Brown-Forsythe and Welch ANOVA tests ( ii ). Scale bars = 30 µm.

Article Snippet: After primary Ab incubations, samples were washed three times with 0.5 % Tween-20 prior to addition of secondary Ab solutions (diluted appropriately in 1% BSA) and incubation at room temperature in the dark for 1 h. Samples were then washed three times with 0.5 % Tween-20 before mounting onto glass slides using VECTASHEILD antifade mounting medium with DAPI (Vector Laboratories).

Techniques: Immunostaining, Expressing, Derivative Assay

Journal: bioRxiv

Article Title: Multiomic Analysis Reveals Molecular Pathways Associated with Intestinal Aggregation of α-Synuclein

doi: 10.1101/2025.08.27.672706

Figure Lengend Snippet: (A) Putative dopamine biosynthesis pathway within EECs. (B) Representative images of fixed STC-1 cells incubated for 24 h with 50 mM nitrate, 50 mM nitrite, and/or 0.01 mM benserazide (Benz), probed with anti-α-syn aggregate primary antibody MJFR-14. α-Syn aggregate signal is in green, and DAPI-stained nuclei are in blue. (C) Mean fluorescence intensity per cell was quantified from maximum intensity projections acquired by structured illumination microscopy ( n = 3 independent biological replicates, 3–4 technical replicates for each, bars denote mean ± S.E.M.; significance determined by one-way ANOVA with Sidak’s multiple comparison test, ****: P < 0.0001, ***: P = 0.0002).

Article Snippet: After washing three times, ∼10 drops of VECTASHEILD PLUS Antifade Mounting Medium with DAPI (Vector Laboratories) were added to each well.

Techniques: Incubation, Staining, Fluorescence, Microscopy, Comparison